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Restriction fragment length polymorphism

Synonym(s)

  • RFLP
  • RFLP analysis

Definition(s)

Fragment of DNA of predictable size resulting from digestion (cutting) of a strand of DNA by a given restriction enzyme. DNA sequence alterations (mutations) that destroy or create the sites at which a restriction enzyme cuts DNA change the size (and number) of DNA fragments resulting from digestion by a given restriction enzyme.

Definition from: GeneReviewsThis link leads to a site outside Genetics Home Reference. from the University of Washington and the National Center for Biotechnology Information

Variation between individuals in DNA fragment sizes cut by specific restriction enzymes; polymorphic sequences that result in RFLPs are used as markers on both physical maps and genetic linkage maps. RFLPs usually are caused by mutation at a cutting site.

Definition from: Human Genome Project InformationThis link leads to a site outside Genetics Home Reference. at the U.S. Department of Energy

Restriction fragment length polymorphism (RFLP) is a type of polymorphism that results from variation in the DNA sequence recognized by restriction enzymes. These are bacterial enzymes used by scientists to cut DNA molecules at known locations. RFLPs (pronounced "rif lips") are used as markers on genetic maps. Typically, gel electrophoresis is used to visualize RFLPs.

Definition from: Talking Glossary of Genetic TermsThis link leads to a site outside Genetics Home Reference. from the National Human Genome Research Institute

Variation in the length of a restriction fragment produced by a specific restriction enzyme acting on DNA from different individuals that usually results from a genetic mutation (as an insertion or deletion) and that may be used as a genetic marker -- called also RFLP.

Definition from: Merriam-Webster's Medical DictionaryThis link leads to a site outside Genetics Home Reference. by Merriam-Webster Inc.

See also Understanding Medical Terminology.

 
Published: December 16, 2014